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References: <[log in to unmask]>
Content-Type: text/plain; charset=ISO-8859-1
User-Agent: Internet Messaging Program (IMP) 3.2.1
Message-ID:  <[log in to unmask]>
Date:         Mon, 19 Jul 2004 10:02:38 -0500
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         "Dirk J. Andres" <[log in to unmask]>
Subject:      Re: Subject: ImageJ appropriate for evaluation of Thin
              LayerChromatopgraphy ?
In-Reply-To:  <[log in to unmask]>

My name is Dirk Andres and I am currently doing my undergraduate research at
IUPUI in Indianapolis, IN.  I need to know how ImageJ can help me calculate the
area of an image (Neurites!).  In short, what pluggin might I need and how
would I install it for use in ImageJ?  Thanks





Quoting Martin Bloss <[log in to unmask]>:

> ImageJ – appropriate for qualitative evaluation of Thin Layer
> Chromatographie-plates ?
>
> Does ImageJ contain such a function up to now ?
>
> I want to  make a qualitative evaluation of Thin Layer Chromatographie
> Plates as JPG-picture with different colored spots. The colour, geometric
> appearance (spot, zone (from begin-till end), shadows and Rf-value should
> be shown in “Results table”.
>
>
> I know the approach within ImageJ to evaluate Gel-pictures,
> but in gels the Rf-Value is not of interest and the gel-
> approach is made in gray,
> for my application that would mean  the colored spots, zones,
> shadows (I have to indicate)partly would  disappear.
>
> My approach in ImageJ up to now:
>
> Open jpg-Picture
> Select the rectangular ROI, and then cover the thin
> layer chromatogramm from Start-line upwards (important
> otherwise the lengths calibration doesn´t work correct).
>
> Click Function  “crop “, that delivers the colored
> picture I want to evaluate.
> Click “measurements” and select “ Invert- Y-coordinates”
> and “Display-Label.”
> Click  “Straight line selection” and pull (push shift,
> produces a vertikal line)) inside picture a line from
> start to end of lane.
> Click “Set scale “and fill in” Known distance = 1.00 “and
> in “Unit of lengths  = Rf “ .
> Select” cross hair tool (mark and count)”and click the
> spots inside the lane.
> The results table shows:
>
> Nr of measurement, Label, X, Y, value
>
> I only need Label and Y(Rf-Values), how can I remove the other columns ?
>
> How can I rename Y-Column in Rf?
>
> How can I generate a further Column to insert the colour of the spots ?
>
>  To indicate a zone that begins I click the” Comment-writer” and
> insert "Zone-Start at Rf = "
>
> followed by the two measurments (start and End of zone) and once again I
> click the “Comment-writer”  and insert "Zone-End."
>
> How can I automate this?
>
> I there a way to get the measurements instantly in
> Excel, maybe I there could add the the color of the spot.
>
> Many question, maybe someone can support  some
> information, advise about that. Maybe there is a more
> elegant way to solve this.
>
> Many thanks for endurance reading this.
> I look forward to hear some statements. Thanks in advance.
>
> Best regards
>
> Martin Bloss, Germany
>
>
>

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