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Message-ID:  <001c01c46c3d$6355d1b0$ce44cad4@namexv69u5dbyu>
Date:         Sat, 17 Jul 2004 22:33:57 +0200
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         Martin Bloss <[log in to unmask]>
Subject:      Appropriate for evaluation of Thin LayerChromatopgraphy ?

----- Original Message ----- 
From: Martin Bloss 
To: [log in to unmask] 
Sent: Saturday, July 10, 2004 8:49 PM
Subject: Appropriate for evaluation of Thin LayerChromatopgraphy ?


 

ImageJ - appropriate for qualitative evaluation of Thin Layer Chromatographie-plates ?

 

Does ImageJ contain such a function up to now ?

 

I want to  make a qualitative evaluation of Thin Layer Chromatographie Plates as JPG-picture with different colored spots. The colour, geometric appearance (spot, zone (from begin-till end), shadows and Rf-value should be shown in "Results table".

 

 I know the approach within ImageJ to evaluate Gel-pictures, 

but in gels the Rf-Value is not of interest and the gel- approach is made in gray,

 for my application that would mean  the colored spots, zones, shadows (I have to indicate) would partly disappear. 

 

My approach in ImageJ up to now:

  1.. Open jpg-Picture 
  2.. Select the rectangular ROI, and then cover the thin layer chromatogramm from Start-line upwards (important otherwise the lengths calibration doesn´t work correct). 
  3.. Click Function  "crop ", that delivers the colored picture I want to evaluate. 
  4.. Click "measurements" and select " Invert- Y-coordinates" and "Display-Label." 
  5.. Click  "Straight line selection" and pull (push shift, produces a vertikal line)) inside picture a line from start to end of lane. 
  6.. Click "Set scale "and fill in" Known distance = 1.00 "and in "Unit of lengths  = Rf " . 
  7.. Select" cross hair tool (mark and count)"and click the spots inside the lane.
The results table shows:

Nr of measurement, Label, X, Y, value

 

I only need Label and Y(Rf-Values), how can I remove the other columns ?

 

How can I rename Y-Column in Rf?

 

How can I generate a further Column to insert the colour of the spots ?

 

To indicate a zone that begins I click the" Comment-writer" and insert Zone-Start at Rf =

followed by the two measurments (start and End of zone) and once again I click the "Comment-writer" Zone-End. 

How can I automate this?

 

I there a way to get the measurements instantly in Excel, maybe I there could add the the color of the spot.

 

Many question, maybe someone can support  some information, advise about that. Maybe there is a more elegant way to solve this.  Many thanks for endurance reading this. I look forward to hear some statements. Thanks in advance.

 

Best regards 

 

Martin Bloss, Germany

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