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Message-ID:  <004201c35d2c$81b17220$08fda8c0@kinase>
Date:         Thu, 7 Aug 2003 17:40:22 -0400
Reply-To:     [log in to unmask]
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         Milton Charlton <[log in to unmask]>
Organization: University of Toronto
Subject:      Time lapse image acquisition plugin?
In-Reply-To:  <[log in to unmask]>

Does anyone have a plug-in for Image J that will acquire time lapse images
at set intervals from a standard Windows imaging frame grabber such as an
ATI All-In -Wonder or from a USB "Webcam"?  We need to write all the images
collected to a continuous file for analysis by Image J.  Currently we use
WinTLV (with the ATI All-In-Wonder) to take time lapse images and make an
AVI file that we decompress  with VirtualDub and then get Image J to eat the
result for analysis.   I want to minimize the number of programs required to
carry out an investigation.
I would also like to have online graphing of intensity changes in areas of
interest as new frames are acquired, i.e.. a graph system that expands the X
axis (time) as new measurements are added during an experiment.  This means
the system would have to store each new image, make a measurement(s) on it
and put a new point(s) on the graph.  Axon Imaging Workbench (now Imaging
Workbench sold by Indec) does this very well.  I realize that is a tall
order for a plug-in but perhaps one could ship the measurement to an
independent graphing program in another window.  The time lapse interval is
greater than 500ms so there is quite a bit of time to do this.
Thanks for your help.

Milton P. Charlton
Physiology Department
University of Toronto

tel:  416-978-6355
fax:  416-978-4940
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