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Message-ID:  <[log in to unmask]>
Date:         Tue, 5 Aug 2003 20:12:20 -0700
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         Changpeng Li <[log in to unmask]>
Subject:      Re: About the nonuniformity of brightness.
In-Reply-To:  <[log in to unmask]>

I try to subtract background. It really helps to get
the uniform brightness distribution. Thanks for your
kind suggestion. BTW, the entered radius is the pixel
number, right?
However, I also find that some noise happend (black
speckles on the white background) after the substract
of background. Do you now how to lessen or remove
these speckles?
Thanks for your kind help!

Changpeng

--- GC <[log in to unmask]> wrote:
> Try with Process/Substract background... before
> segmentation. The entered
> radius must be larger than the radius of the dots.
>
> Gary.
>
>
> ----- Original Message -----
> From: "Changpeng Li" <[log in to unmask]>
> To: <[log in to unmask]>
> Sent: Tuesday, July 29, 2003 7:38 PM
> Subject: About the nonuniformity of brightness.
>
>
> > Dear Sir/Madam:
> >     I get some images about the dot structure
> using
> > SEM. However, because these dots are not at the
> same
> > level or some other reasons, they exhibits
> nonuniform
> > brightness distribution. In this way, the dots in
> the
> > darker parts shows much larger diameter than those
> in
> > the brighter parts (the image is composed of black
> > dots on the white background). Even worse,
> sometimes
> > when I want to make sure the dots in the darker
> parts
> > seperated each other, some of the dots in the
> brighter
> > parter will disappear.
> >     I have been stuck by this problem for a long
> > period for the reasonable distribution
> measurement.
> > Your suggestion is really appreciated.
> >     Thank you for your kind consideration!
> >
> >    Changpeng
> >
> > __________________________________
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> >
> >


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