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Date:         Mon, 11 Aug 2008 10:59:03 -0700
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         elambeth <[log in to unmask]>
Subject:      Excluding black (255) from an image

I am working with red stained images of frog liver.  There are pigment cells
in the liver that are black, and should not count for the quantification of
the red stain.  I want to know the mean gray value of the red pixels, and I
know this is on the histogram, but I do not know how to exclude the black
pigment cells from the histogram (basically I want a histogram of the red
stain only).  Is there any way to exclude the black?

Emily Lambeth, Graduate Student
Department of Biological Sciences
Notre Dame University

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