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Approved-By: [log in to unmask]
Message-ID:  <[log in to unmask]>
Date:         Thu, 6 Dec 2007 10:47:34 -0500
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         Aaron Johnson <[log in to unmask]>
Subject:      Measuring Volume

Hello, I'm interested in finding a way to do a volume measurement of an 8-
bit grayscale stack (usually between 15-20 images) coming from a confocal 
microscope.  The structure I'm measuring (ACh receptor) is fairly 
contiguous and could be characterized as blob-shaped.  My main questions 
are:

1) What are your recommendations regarding thresholding?  I always want to 
capture the max (255), and am more concerned about finding an automated 
way of finding the minimum.  The "auto-threshold" doesn't work for me.

2) What do you recommend for the volume measurement?  I've tried voxel 
counter with some success, but I'm wondering if there are other easy ways 
to do this, if for no other reason than to confirm my measurements with 
voxel counter.

Thanks!
Aaron

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