Previous Message | Next Message Previous in Topic | Next in Topic Previous by Same Author | Next by Same Author Previous Page (December 2007) | Back to Main IMAGEJ Page
	Reply | Post a New Message | Join or Leave IMAGEJ, or Change Options | Search
	Chronologically | Most Recent First | Wrap Text (Proportional Font) | Don't Wrap Text (Non-proportional Font)

Approved-By: [log in to unmask]
Content-Language: en
Priority: normal
Content-Type: text/plain; charset=us-ascii
Content-Disposition: inline
Message-ID:  <[log in to unmask]>
Date:         Mon, 10 Dec 2007 16:16:02 -0500
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         ALEX CHAMPAGNE <[log in to unmask]>
Subject:      Baseline for Gel Analysis2

Thanks to the people who sent me suggestions regarding the subtract background function.  I've tried that function before, and I'm not satisfied with the results.  Often, even when the background has been subtracted, tails still exist, and it becomes an arbitrary process to draw lines that "should" bound the peak.  In this way, the methods can not be standardized, and depending on where the lines are placed, the precise result varies each time.  Is there any plugin which might standardize the designation of the peaks and/or provide a baseline better than the current subtract background function?

Thanks,
Alex Champagne
Ohio State University

Center for Information Technology National Institutes of Health Bethesda, Maryland 20892 301 594 6248 (v) 301 496 8294 (TDD) Comments and Assistance Accessibility