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References:  <[log in to unmask]>
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Message-ID:  <089801c1fc1b$8d5f8ad0$6401a8c0@argonaut>
Date:         Wed, 15 May 2002 07:19:40 -0700
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         "Harvey J. Karten" <[log in to unmask]>
Organization: UCSD

I don't know of a specific tool to do that. But what I have done is to split
the stack into three stacks. I then made a Z-projection of each of the three
stacks, producing three separate images. I then assigned them as a Red,
Green and Blue planes, respectively, and displayed them as an RGB image. The
most distal plane of dendrites was shown in red, the intermediate plane of
dendrites in green and the most proximal in blue.

I'm sure that a simple interactive macro could be written to accomplish
this. The major point requiring user interaction would be deciding where to
draw the boundaries in the stack for those sections intended to be red, vs.
those to be green, etc.

The tool you propose would even potentially be more valuable for certain
applications, as it would show a continuous color grading based on depth.
For purposes of visualizing the depth location of sharply laminarly
distributed dendrites, our current strategy provides clear images. But a
macro would greatly facilitate this operation.
regards,
Harvey Karten
Dept. Neurosciences
UCSD
La Jolla, CA

----- Original Message -----
From: "Nathalie Van Bruaene" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, May 15, 2002 6:41 AM


Dear all,

is there a tool available to make a colour coded z-projection of a stack,
where the colour indicates the z-depth?

Thanks!

Nathalie
------------------------------------------------------------------
Nathalie Van Bruaene

Ghent University
Faculty of Agricultural and Applied Biological Sciences
Lab. Biochemistry and Molecular Cytology
Coupure Links 653, B-9000 Gent, Belgium
Tel: 32 (0)9 264 59 71
Fax: 32 (0)9 264 62 19

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