Previous Message | Next Message Previous in Topic | Next in Topic Previous by Same Author | Next by Same Author Previous Page (May 2002) | Back to Main IMAGEJ Page
	Reply | Post a New Message | Join or Leave IMAGEJ, or Change Options | Search
	Chronologically | Most Recent First | Wrap Text (Proportional Font) | Don't Wrap Text (Non-proportional Font)

References: <[log in to unmask]>
Content-Type: text/plain; charset="iso-8859-1"
Message-ID:  <00eb01c1f8ca$9f605f60$730dc4d5@server>
Date:         Sat, 11 May 2002 11:02:47 +0200
Reply-To:     "Dr. Michael D. Abramoff" <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         "Dr. Michael D. Abramoff" <[log in to unmask]>
Subject:      Re: interference filter plugin

I agree that it would be very useful to have an SI (Spectrum/Intensity) 'image' conversion function. You could then use a kind of specrtal thresholding to do what you want to do. Foley (2nd edition) Computer Graphics stated that there is a n-to-1 mapping of spectral distribution to color, which may complicate the design of such a function.
I imagine it has been tackled in industrial image recognition (differentiating good/bad apples by color spectrum, etc.)
I hope you hear about something useful somewhere. I am interested, but right now am putting all my spare time into my real-time renderer.

Michael Abramoff

_____________________________________________________________________________________
Michael Abramoff, MD, PhD
Dept. of Ophthalmology, Room 4A65,
Vrije Universiteit University Hospital, 1117, de Boelelaan, 1080 MB Amsterdam, The Netherlands
[log in to unmask]
Image Sciences Institute, Room E01.334
University Medical Center Utrecht, 100, Heidelberglaan, 3584 CX Utrecht, The Netherlands
[log in to unmask]
_____________________________________________________________________________________




----- Original Message ----- 
  From: F Javier Diez Guerra 
  To: [log in to unmask] 
  Sent: Saturday, May 11, 2002 9:59 AM
  Subject: interference filter plugin


  Dear Martin,

  I am not looking for a plugin that substitutes for a physical filter.

  Imagine that you have captured beautiful fluorescence frames from
  GFP-fusioned protein with a color camera. In addition to bright green
  stained cells, you also observe that most cells have an orange-yellowish
  staining because of a treatment with a protein kinase inhibitor that shows
  some fluorescence.

  In order to remove the orange background staining, I understand that the
  best approach is to use a narrow-band emission filter in the cube. This is
  something that we definitely will try once we get such filter.

  In the meantime, we have these beautiful time-lapsed images that we would
  like to enhance. If the background color were red instead of orange, we all
  would consider appropriate to separate RGB channels and then pick the green
  one. However, RGB channel separation does not render proper background
  removal in this case.

  So my question:
  Is there any way (or plugin) of post-processing such images to remove the
  orange background while maintaining the green signal?

  Thanks,

Center for Information Technology National Institutes of Health Bethesda, Maryland 20892 301 594 6248 (v) 301 496 8294 (TDD) Comments and Assistance Accessibility