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Message-ID:  <[log in to unmask]>
Date:         Sat, 11 May 2002 09:59:28 +0200
Reply-To:     ImageJ Interest Group <[log in to unmask]>
Sender:       ImageJ Interest Group <[log in to unmask]>
From:         F Javier Diez Guerra <[log in to unmask]>
Subject:      interference filter plugin

Dear Martin,

I am not looking for a plugin that substitutes for a physical filter.

Imagine that you have captured beautiful fluorescence frames from
GFP-fusioned protein with a color camera. In addition to bright green
stained cells, you also observe that most cells have an orange-yellowish
staining because of a treatment with a protein kinase inhibitor that shows
some fluorescence.

In order to remove the orange background staining, I understand that the
best approach is to use a narrow-band emission filter in the cube. This is
something that we definitely will try once we get such filter.

In the meantime, we have these beautiful time-lapsed images that we would
like to enhance. If the background color were red instead of orange, we all
would consider appropriate to separate RGB channels and then pick the green
one. However, RGB channel separation does not render proper background
removal in this case.

So my question:
Is there any way (or plugin) of post-processing such images to remove the
orange background while maintaining the green signal?

Thanks,

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