von Laer D, Hufert F, Kern P, Schwander S, Fenner T, Dietrich M, Schmitz H; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 151 (abstract no. F.A.300).
Bernhard-Nocht-Institute of Tropical Medicine, Clinical and Virological Department, Hamburg, F.R.G.
OBJECTIVE: HIV-1 infection is frequently associated with anemia and leukopenia. The replication of HIV in bone marrow stem cells has been proposed as a potential explanation. METHODS: To study this hypothesis we purified CD34+ cells (which are highly enriched in hematopoietic progenitors) from the bone marrows of 14 HIV-infected individuals (12 patients with AIDS and 2 with ARC. 10 with blood cytopenias) using a Fluorescence Activated Cell Sorter (FACS). As internal controls CD4+ lymphocytes, monocytes (CD14), granulocytes and CD4- lymphocytes were sorted. A polymerase chain reaction (PCR) was then performed using 2 different primer pairs (env and gag region) to detect HIV in the purified cell populations. RESULTS: HIV genome was only detected in the CD34+ cells of one bone marrow sample. The CD4+ lymphocytes of all samples were infected with HIV, while only few individuals carried the virus in the bone marrow monocytes. CONCLUSION: These studies show that the blood cytopenias observed in HIV infection cannot simply be explained by the infection of hematopoietic progenitor cells.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens, CD34
- Bone Marrow
- Bone Marrow Cells
- CD4-Positive T-Lymphocytes
- Genes, gag
- HIV
- HIV Infections
- HIV Seronegativity
- HIV Seropositivity
- HIV-1
- Hematopoiesis
- Hematopoietic Stem Cells
- Humans
- Monocytes
- Polymerase Chain Reaction
- genetics
- immunology
Other ID:
UI: 102184203
From Meeting Abstracts