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Completion of a Comprehensive Mouse Knockout Resource
Release Date: September 8, 2005
Announcement Number: RFA-HG-05-007
Application Receipt Date:
November 22, 2005
Letter of Intent Date: October 20, 2005
Funding Contact: Robert Riddle, Ph.D.
Program Area: Neurogenetics
Also See:
http://grants.nih.gov/grants/guide/notice-files/NOT-HG-06-001.html
Brief Description:
The purpose of this RFA is to solicit applications for research projects to initiate a trans-NIH program for the production
of a comprehensive resource of mouse mutants in which every gene in the mouse genome has been knocked out by a null mutation
marked with a reporter system of high utility. The ideal resource would be one in which all of the mutations are carried on
a uniform background in strain C57BL/6, which is the strain most widely utilized by mouse researchers. But the NIH recognizes
that, at present, there are certain technical problems, primarily related to low efficiency, with the use of C57BL/6 as the
background strain for this type of high-throughput project. Therefore, the primary focus of this solicitation will be on construction
of the mutations, and a secondary focus will be on the choice of mouse stain. However, applications that propose approaches
to the cost-efficient generation of the mutations directly in strain C57BL/6 will be given preference for funding.
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