Wong JT, Ablikim M, Wang Z, Liu Z; Conference on Retroviruses and Opportunistic Infections.
Abstr 10th Conf Retrovir Oppor Infect Feb 10 14 2003 Hynes Conv Cent Boston Mass USA Conf Retrovir Oppor Infect 10th 2003 Boston Mass. 2003 Feb 10-14; 10: abstract no. 301.
Harvard Med Sch and Mass Gen Hosp, MA
BACKGROUND: Effective amplification of the response of CD4+ T-cells depends on both the proliferative capacities of the CD4+ T-cells upon antigen stimulation and the immunologic functions of the proliferating cells through the different stages of proliferation.METHODS: Proliferative capacities of the peripheral blood CD4+ T-cells from 5 non-HIV infected controls and 6 HIV-infected subjects were studied using an anti-CD3:anti-CD8 bispecific mAb (CD3,8) + IL2 + HAART in an artificial capillary cartridge system. CD3,8 was left in for the first 4 days and HAART was left in for the first 19 days only. Proliferating cells were partially harvested periodically and frozen. The cells from early and late stages of proliferation were thawed together and immunologic assays performed.RESULTS: CD3,8 redirect cytolysis of CD8+ T-cells and led to proliferation of the residual CD4+ T-cells by stimulating the TCR in the presence of IL2. In the presence of HAART, CD3,8 + IL2 led to elimination of replication-competent virus presumably by activating the latent virus to lyse the infected cells while preventing new infections from being established. For normal subjects, the number of doubling achieved varied between 17.3 to > 38.1 generations (25.1 mean). For HIV-infected subjects, the number of doubling achieved varied between 10 to 38 (19.7 mean). For 3 subjects whose CD4+ T-cell count was
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antigens, CD3
- Antigens, CD4
- Antigens, CD8
- Antiretroviral Therapy, Highly Active
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- HIV Infections
- HIV Seropositivity
- Immunocompetence
- Interleukin-2
- Receptors, Antigen, T-Cell
- T-Lymphocyte Subsets
- T-Lymphocytes
- immunology
Other ID:
UI: 102260885
From Meeting Abstracts