Yamamura Y, Rodriguez N, Alvarado W, Yano S, Yano N; National Conference on Human Retroviruses and Related Infections.
Program Abstr First Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 1st 1993 Wash DC. 1993 Dec 12-16; 112.
Ponce School of Medicine AIDS Research Program, Ponce, PR.
An unusual sub-population of CD8 T cells were identified in HIV-1(+) subjects; which killed CD4 cells from both HIV-1(+) (hivCD4) and normal (nCD4) subjects. By utilizing a cell- linker, PKH-26 (Zynaxis), the mechanism of this unusual CTL activity was analyzed by flow cytometry. In mitogen activated culture of mononuclear cells (MNC) from HIV-1(+) subjects, hivCD4 were largely destroyed while hivCD8 vigorously proliferated; and addition of IL2 did not abrogate hivCD4 death. Such hivCD4 death was not observed in unstimulated cultures. Mitogenic stimulation of purified hivCD4 did not induce significant cell death; and the proliferation profile of purified hivCD4 was similar to that of nCD4 in either nMNC or purified nCD4 cultures. Addition of nCD8 to hivCD4 did not affect either the survival or proliferation profile of the latter cells; while addition of hivCD8 to nCD4 destroyed the latter. This "promiscuous CTL" (proCTL) activity was directed somewhat preferentially to CD4 cells with slower replication and those CD4 cells which proliferated more rapidly were affected to much lesser extent. ProCTL activity was less frequent in asymptomatic subjects with higher CD4 counts, but was more frequent and more pronounced in subjects with progressive disease. It is thus suggested that the proCTL may be directly responsible for a decline of CD4 T cells often associated with the disease progression.
Publication Types:
Keywords:
- Antigens, CD4
- Antigens, CD8
- CASP4 protein, human
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- Caspases
- Flow Cytometry
- HIV-1
- Humans
- Interleukin-2
- Resin Cements
- T-Lymphocytes
- T-Lymphocytes, Cytotoxic
- immunology
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102214283
From Meeting Abstracts