Mass Spectrometry
Additional Information
EMSL's mass spectrometry capabilities enable high-throughput, high-resolution analysis of complex mixtures. These resources are applied to a broad range of scientific problems from proteomics studies with applications to human health and environmental remediation to aerosol particle characterization, as well as fundamental studies of ion-surface collisions and preparatory mass spectrometry using ion soft-landing.
Proteomics Resources and Research
Proteomics capabilities housed in EMSL, including state-of-the-art instruments and sophisticated bioinformatics methods, have been developed and acquired in coordination with the EMSL scientific user program and Pacific Northwest National Laboratory (PNNL) programmatic research. These resources may be accessed via EMSL User Access or by collaborating with scientists who perform proteomics research at PNNL.
Cutting-edge proteomics tools and methods available at EMSL facilitate advanced global proteomics research and allow detailed visualization and analyses of cellular proteins. Proteomics research and tools at EMSL include:
- Cell signaling and responses at the molecular level to chemical or radiological insults
- Proteomic analyses of whole cell lysates
- Analyses of organic macromolecules and protein complexes
- Targeted proteomic analyses of subcellular fractions
- Top-down proteomics methods
- Quantitation using isotopic labeling and label-free approaches.
Though access to mass spectrometry hardware at EMSL is only available to EMSL users and PNNL collaborators, PNNL and EMSL provide a collection of software tools to the entire proteomics community through the National Center for Research Resources Proteomics Research Resource for Integrative Biology, such as the PC-based software package, DECON2LS, and VIPER, which offers 'omics data analysis capabilities. Software and data can also be accessed via Biological and Environmental Research – PNNL Proteomics.
Aerosol Particle Characterization Resources and Research
Unique, state-of-the-art mass spectrometry tools enable EMSL users to characterize environmentally relevant aerosols with high specificity and resolution. Scientists use these tools to study fast chemical reactions; characterize particles with low number concentrations; detect and characterize the chemical structure of high-molecular weight organic compounds in aerosol and cloud water samples; and characterize in detail the chemical composition and transformation of particulate matter collected on substrates using novel atmospheric pressure surface ionization techniques.
Many EMSL aerosol characterization instruments are field deployable, allowing researchers to capture aerosol data on location, in real time. Other instruments are used for comprehensive off-line characterization of atmospheric aerosols.
ION-SURFACE COLLISIONS RESOURCES AND RESEARCH
EMSL hosts a number of unique mass spectrometry instruments specially configured for studying fundamental aspects of activation, dissociation, and deposition (soft-landing) of complex molecular ions following collision with specially prepared surfaces. EMSL researchers use a combination of experimental studies of collisional activation and dissociation of complex molecules in a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer with RRKM modeling to determine the internal energy distribution of excited ions and the energetics and dynamics of dissociation of a variety of complex ions and non-covalent complexes. Preparatory mass spectrometry using soft-landing of mass selected ions on surfaces enables highly specific modification of surfaces, preparation of novel materials, and facilitates molecular level understanding of interactions of biomolecules with hydrophilic and hydrophobic surfaces in living systems.
Capability Detail
Refer to the table below for a full listing, which leads to complete information about each of EMSL's mass spectrometry instruments. Brief details about the primary mass spectrometry tools available to EMSL users immediately follow.
Proteomics Capabilities
- Fourier-transform mass spectrometers, including 7-T, 9.4-T, 11.5-T, and 12-T FTICR spectrometers; one 7-T linear ion trap quadrupole-FT (LTQ-FT) spectrometer; and three Orbitraps
- Micromass quadrupole time-of-flight QTOF mass spectrometer, with custom high-sensitivity inlet, equipped with a microspray source
- Five ion trap spectrometers, four linear ion trap mass spectrometers, and a triple-quadrupole spectrometer
- Fifteen custom high-performance liquid chromatography systems
- Three Agilent capillary HPLC systems
Aerosol and Ion-Surface Collision Capabilities
- LTQ Orbitrap for environmental research
- Field-deployable second-generation single-particle laser-ablation time-of-flight mass spectrometer
- 6-Tesla FTICR specially configured for studying ion-surface interactions
- Ion deposition instrument for selective preparation of novel materials using ion soft-landing
All Related Publications Related Publications
- Oxygen-dependent autoaggregation in Shewanella oneidensis MR-1.
- Apoferritin-Templated Yttrium Phosphate Nanoparticle Conjugates for Radioimmunotherapy of Cancers.
- Comparative Proteomics of Human Monkeypox and Vaccinia Intracellular Mature and Extracellular Enveloped Virions.
- Chemical Speciation of Sulfur in Marine Cloud Droplets and Particles: Analysis of Individual Particles from the Marine Boundary Layer Over the California Current.
- Reactive Landing of Peptide Ions on Self-Assembled Monolayer Surfaces: A Alternative Approach for Covalent Immobilization of Peptides on Surfaces.
Related Research Highlights
- Energetics and Dynamics of Electron Transfer and Proton Transfer in Dissociation of MetalIII(salen)-Peptide Complexes in the Gas Phase (Getting a Charge)
- Researchers Utilize Proteomics to Reveal a Core Proteome (Bacteria at the Core)
- A New Mechanism for Ozonolysis of Unsaturated Organics on Solids: Phosphocholines on NaCl as a Model for Sea Salt Particles (Up in the Air)
Mass Spectrometry Capabilities Available at EMSL
Instrument | Contact |
---|---|
Analytical: Inductively Coupled Plasma-Mass Spec (ICP-MS) |
Wietsma, Tom |
Mass Spectometer: Fourier-Transform |
Anderson, David J Moore, Ron Tolic, Ljiljana Pasa |
Mass Spectrometer: FT-ICR, 6 Tesla |
Laskin, Julia |
Mass Spectrometer: Ion trap |
Moore, Ron Tolic, Ljiljana Pasa |
Mass Spectrometer: Laser Desorption - Ion Trap |
Alexander, M Lizabeth |
Mass Spectrometer: Quadrupole TOF |
Anderson, David J |
Mass Spectrometer: Aerosol - time-of-flight |
Alexander, M Lizabeth |
Mass Spectrometer: Isotope Ratio |
Alexander, M Lizabeth |
Mass Spectrometer: Linear Ion Trap Quadrupole (LTQ) Orbitrap MS - for environmental research |
Laskin, Alexander |
Mass Spectrometer: Single Particle -(SPLAT II) |
Zelenyuk, Alla |
Mass Spectrometer: Time of Flight Secondary Ion (ToF SIMS) - 1997 |
Laskin, Julia |
Mass-Selected Ion Deposition System - Electrospray Source |
Laskin, Julia |
Proton Transfer Reaction Mass Spec(PTRMS) |
Alexander, M Lizabeth |
Mass Spectrometry Capability Steward (Aerosols): Roy Gephart | roy.gephart@pnl.gov, 509-371-6142
Mass Spectrometry Capability Steward (Materials): Theva Thevuthasan | theva@pnl.gov, 509-371-6244