Award Abstract #0233971
Continuous Optical Fractionation of Biological Materials

NSF Org:	DBI Division of Biological Infrastructure
Initial Amendment Date:	May 22, 2003
Latest Amendment Date:	September 1, 2004
Award Number:	0233971
Award Instrument:	Continuing grant
Program Manager:	Gerald Selzer DBI Division of Biological Infrastructure BIO Directorate for Biological Sciences
Start Date:	June 1, 2003
Expires:	June 30, 2006 (Estimated)
Awarded Amount to Date:	$553113
Investigator(s):	David Grier david.grier@nyu.edu (Principal Investigator)
Sponsor:	University of Chicago 5801 South Ellis Avenue Chicago, IL 60637 773/702-8602
NSF Program(s):	INSTRUMENTAT & INSTRUMENT DEVP
Field Application(s):
Program Reference Code(s):	BIOT,9184
Program Element Code(s):	1108

ABSTRACT

This award supports development of a compact, integrated system for continuous separation of fluid-borne materials such as macromolecules, nanoclusters, colloidal particles, cell fragments and entire living cells. The device is based on optical fractionation, a recently discovered strategy based on differing interactions with light to distinguish and sort specified subpopulations of particles - for example, cells of differing size - within heterogeneous samples (complex mixtures). The device uses arrays of optical traps to selectively deflect materials into microfluidic channels for collection and processing. The basic principle has been demonstrated on the laboratory scale. The current grant will support (1) development of the technology into a less expensive and more compact, turn-key system, and (2) development of protocols for optically fractionating different classes of biomaterials. The optical fractionation instrument offers continuous sample sorting and separation with an unprecedented combination of accuracy, generality, ease of use and cost-effectiveness. Consequently, it should find widespread applications in biological research, ranging from laboratory purification of both synthetic and natural biomolecules to new modes of cell cytometry. In addition to development of the instrument, the project is expected to provide the basis for two doctoral theses and three undergraduate honors theses in biophysics.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

(Showing: 1 - 13 of 13).

B. A. Koss and D. G. Grier.  "Optical peristalsis,"  Applied Physics Letters,  v.82,  2003,  p. 3985.

D. G. Grier.  "A revolution in optical manipulation,"  Nature,  v.424,  2003,  p. 810.

D. G. Grier and E. R. Dufresne.  "Method for applying optical gradient forces and moving material,"  U. S. Patent 6,624,940,  2003,  p. 1.

D. G. Grier and S. H. Behrens.  "Optical peristaltic pumping with optical traps,"  U. S. Patent 6,639,208,  2003,  p. 1.

D. G. Grier, E. R. Dufresne, J. E. Curtis and B. A. Koss.  "Apparatus for using optical tweezers to manipulate materials,"  U. S. Patent 6,626,546,  2003,  p. 1.

J. E. Curtis and D. G. Grier.  "Structure of optical vortices,"  Physical Review Letters,  v.90,  2003,  p. 133901.

J. E. Curtis and D. G. Grier.  "Modulated optical vortices,"  Optics Letters,  v.28,  2003,  p. 872.

K. Ladavac and D. G. Grier.  "Colloidal hydrodynamic coupling in concentric optical vortices,"  Europhys. Lett.,  v.70,  2005,  p. 548.

M. Polin, K. Ladavac, S. Lee, Y. Roichman and D. G. Grier.  "Optimized holographic optical traps,"  Optics Express,  v.13,  2005,  p. 5831.

S. Lee and D. G. Grier.  "Flux reversal in a two-state optical thermal ratchet,"  Phys. Rev. E,  v.71,  2005,  p. 060102(R).

S. Lee and D. G. Grier.  "Robustness of holographic optical traps against phase scaling errors,"  Optics Express,  v.13,  2005,  p. 7458.

S. Lee and D. G. Grier.  "One-dimensional optical thermal ratchets,"  J. Phys.: Cond. Matt.,  v.17,  2005,  p. S3685.

S. Lee, K. Ladavac, M. Polin, and D. G. Grier.  "Observation of flux reversal in a symmetric optical thermal ratchet,"  Phys. Rev. Lett.,  v.94,  2005,  p. 110601.


(Showing: 1 - 13 of 13).

Please report errors in award information by writing to: awardsearch@nsf.gov.