Ottinger JS, Gryszowka VE, Huggins JM, Wellons MF, Bartlett JA, Weinhold KJ; Conference on Retroviruses and Opportunistic Infections.

Program Abstr 8th Conf Retrovir Oppor Infect Conf Retrovir Oppor Infect 8th 2001 Chic Ill. 2001 Feb 4-8; 8: 153 (abstract no. 369).

Duke Univ Med Ctr, Durham, NC.

Background; Quantitative changes in markers of cellular activation, such as CD38 and CD95, have been associated with AIDS pathogenesis and disease progression. In this cross-sectional study, expression of the activation markers CD38 and CD95, as well as the chemokine receptors CXCR4 and CCR5, was quantitated on defined CD4+ and CD8+ lymphocyte subsets. Methods: Fresh whole- blood specimens were obtained from uninfected volunteers and HAART-treated HIV-1+ patients categorized as 'stable suppressed' (SS), 'low viral relapse' (LVR), or 'high viral relapse' (HVR). The CD4+ and CD8+ lymphocyte subsets were defined as naive (CD62L+ CD45RA+), memory (CD62L+ CD45RA-, CD62L- CD45RA-, CD62L- CD45RA+), and activated (CD38+ HLA- DR+). Results: CD38 antibodies bound per cell (ABC) increased across all groups for all subsets of CD4+ as well as total and memory CD8+ lymphocytes. Among CD4+ lymphocytes, CD38 ABC was greater on the naive subset, while naive and activated CD4+ cells expressed the most CD38 ABC, irrespective of study group. In contrast, the naive to memory ratio of CD38 ABC on CD8+ subsets decreased across all groups. CD95 ABC increased significantly between the SS and LVR groups for CD4+ and CD8+ total and memory subsets, as well as the naive CD4+ lymphocytes. In addition, CD95 ABC was more significant in the CD4+ subset compared to the CD8+ subset. Within CD4+ cells, CD95 ABC was more profound in the naive subset. For CXCR4 and CCR5, no differences were observed across groups for either CD4+ or CD8+ lymphocyte subsets. Conclusion: In conclusion, quantitative expression of CD38, and perhaps CD95, on naive CD4 subsets may represent a significant immunologic parameter for predicting viral relapse on HAART.

Publication Types:

• Meeting Abstracts

Keywords:

• ADP-ribosyl Cyclase
• Acquired Immunodeficiency Syndrome
• Antigens, CD
• Antigens, CD4
• Antigens, CD45
• Antigens, CD8
• Antigens, CD95
• Antiretroviral Therapy, Highly Active
• Biological Markers
• CD4-Positive T-Lymphocytes
• CD8-Positive T-Lymphocytes
• Cross-Sectional Studies
• HIV-1
• Humans
• Lymphocyte Count
• Lymphocyte Subsets
• Receptors, CCR5
• Receptors, CXCR4
• immunology
• virology

Other ID:

• GWAIDS0006656

UI: 102244152

From Meeting Abstracts