Corbeil J, Gingeras TR, Mamtora G, Huang D, Richman DD; International Conference on AIDS.
Int Conf AIDS. 1998; 12: 10 (abstract no. 380/1139).
University of California, San Diego, La Jolla 92093-0679, USA.
We have explored how high density arrays (DNA chips) could be used to better understand how gene expression in HIV-infected cells is globally altered during the course of infection. Simultaneous mRNA expression levels for the HIV-1 genome and 6640 human genes present in the reporter lymphoblastoid T cell line CEM-GFP were determined. CEM-GFP were infected with HIV-1LAI at a multiplicity of infection of 0.5. RNA was prepared from control and HIV-infected at 2 h, 24 h and 48 h after infection. The percentage of infected and apoptotic cells were also evaluated to correlate with gene expression. Specific genes were identified that were expressed only in HIV infected cells (2 h; 261 of 2299; 24 h: 278 of 1538 and 48 h: 319 of 1576) or in the control (2 h: 362 of 2299; 24 h: 293 of 1538 and 48 h: 322 of 1576). A number of genes were specifically modulated (> 3-10 or > 10 fold) upon infection. TABULAR DATA, SEE ABSTRACT VOLUME. Genes known to be involved in pathways associated with apoptosis induction, G-protein signaling, stress response and cell cycle control were among those modulated upon HIV infection. The strengths of these analyses are their reproducibility, the ability to measure changes globally and the possibility of identifing new interactions.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Apoptosis
- Gene Expression
- Gene Expression Profiling
- Gene Expression Regulation
- Gene Expression Regulation, Viral
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Oligonucleotide Array Sequence Analysis
- T-Lymphocytes
- genetics
Other ID:
UI: 102226673
From Meeting Abstracts