Haubrich RH, Flexner C, Lederman M, Hirsch M, Herman S, Studlack M, Wang J, Dreyer K, Richman DD; National Conference on Human Retroviruses and Related Infections.
Program Abstr Second Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 2nd 1995 Wash DC. 1995 Jan 29-Feb 2; 117.
UCSD San Diego, CA.
OBJECTIVE: To examine baseline and longitudinal RNA levels and to compare these values with other markers of HIV infection, CD4 cells count and p24 antigen. METHODS: Plasma (P) and Serum (S) samples from a subset of patients on a trial comparing a tat antagonist (Ro 24-7429) with nucleoside analogues were analyzed for HIV RNA. Intra-assay variation, differences between S and P, and correlation with CD4 and p24 were analyzed. RESULTS: Duplicate determinations of 93 S samples had a mean RT PCR of 76,676 copies/ml while 93 P samples had a mean of 176,006. The intra-assay coefficient of variation was 25% for S and 30% for P. The correlation between duplicate determinations was .85 for S and .93 for P (p less than .001). Although S values were lower than P, in 43 matched pairs the values were highly correlated (r = .94, p less than .001). There was a significant correlation between baseline P RNA values and baseline p24 levels in 30 matched samples (r = .8, p less than .001). Similarly, changes in P RNA from baseline to weeks 4, 8, and 12 were correlated with changes in p24. The baseline CD4 count was significantly correlated with the log of the RT PCR level. CONCLUSION: Significant correlation between S and P HIV RNA levels were found in this trial. The change in p24 during anti-retroviral therapy was also reflected in the HIV RNA levels.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Antiviral Agents
- Biological Markers
- CD4 Lymphocyte Count
- Case-Control Studies
- HIV Infections
- HIV Seropositivity
- Humans
- Polymerase Chain Reaction
- RNA
- RNA, Viral
- Reverse Transcriptase Polymerase Chain Reaction
- Reverse Transcription
- genetics
Other ID:
UI: 102213317
From Meeting Abstracts