Saltarelli M, Neumann M, Hadziyannis E, Turpin J, Hatzakis A, Shaw G, Graciosi GC, Fauci AS, Pavlakis GN; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 78 (abstract no. WS-B35-1).
University of Athens Medical School, Greece.
We have studied extensively the viral mRNAs produced by different HIV-1 strains in tissue culture and in patient tissues. We have developed a sensitive semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) assay to detect each individual HIV-1 transcript. The RNA-PCR methodology allowed the quantification of all the mRNA species and revealed substantial variability in the expressed viral mRNAs in different viral strains. This methodology allowed the measurement of mRNA expression over time and demonstrated high viral expression levels in primary infection. The HIV-1 transcription profile of sequential isolates obtained from individuals during the viremic stage of the disease have been examined by this method. Initial time points demonstrate high levels of production of all HIV-1 specific transcripts, followed by a sharp reduction in the level of RNA synthesized. The transcriptional level thereafter remains constant through the last available time points. These results indicate that HIV-1-specific transcripts are synthesized in synchrony at low levels throughout the "latent" stage of infection. Peripheral blood mononuclear cells (PBMCs) from individuals at the end stage of disease demonstrate high levels of HIV-1 expression. The transcriptional patterns in PBMCs immediately following infection and in the late stages of the disease are very similar.
Publication Types:
Keywords:
- DNA Primers
- HIV-1
- Humans
- Polymerase Chain Reaction
- RNA Probes
- RNA, Messenger
- Transcription, Genetic
- genetics
Other ID:
UI: 102205731
From Meeting Abstracts