Redfield R, Fowler A, Oster C, Burke DS; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 639 (abstract no. Th.C.P.133).
Walter Reed Army Institute of Research, Washington, DC, USA
OBJECTIVE: Quantitate the minimum number of patient peripheral blood mononuclear cells (PBMC's) required to isolate HIV in culture. METHODS: PBMC's from 12 staged HIV seropositive patients were obtained by Ficoll-Hypaque separation of heparinized blood. Serial ten-fold dilution of cells (3x10(6) to 3x10(2)) were inoculated into cultures of PHA and IL-2 stimulated normal donor target PBMC's. Culture fluids were monitored weekly for 6 weeks for HIV p24 antigen. RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME. HIV was also isolated in cultures from plasma from 5 to the 12 patients (*noted above). Rapid appearance of antigen (less than or equal to 7 days) in cultures using 3x10(6) patient cells correlated significantly (p less than 0.05) with a high titer of circulating infected cells (greater than or equal to 1 in 3x10(3)) and with plasma viremia, and possibly (p = 0.11) with stage of illness. CONCLUSION: Culture-positive blood from HIV patients contains approximately 100 infected cells per cm3, with a range from 10 to 10,000 infected cells per cm3.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Culture
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- Humans
- Phytohemagglutinins
- Viremia
- blood
- immunology
- organization & administration
Other ID:
UI: 102179287
From Meeting Abstracts