Svedersky L, Roey S, Scillian J, Parslow T, Stites D; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 193 (abstract no. Th.A.294).
Department of Laboratory Medicine, UCSF, San Francisco, California, USA
OBJECTIVE: To investigate the use of xenochimeric SCID mice as an in vivo model for human T and B cell immune responses to HIV antigens. METHODS: Male and female SCID mice, 6 to 12 weeks old, were used. One to 3 x 10(7) human peripheral blood lymphocytes, obtained from Ficoll-Hypaque density gradients of HIV seronegative and cytomegalovirus (CMV) antibody positive donors, were injected intraperitoneally. Spleen cells from groups consisting of at least 4 mice were combined and analyzed 1 to 12 weeks post-injection for their ability to proliferate to CMV after 5 days of culture. Splenocytes were analyzed by flow cytometry for the presence of human lymphocyte and monocyte markers. RESULTS: No human monocytes, T or B cells were found when spleen cells from mice injected with human cells 1, 2, 3 or 4 weeks previously were analyzed. When spleen cells from mice injected with human cells 6 to 13 weeks previously were used, a range of 12% to 55% CD4+ cells were found. In these experiments, less than 10% CD8+ and insignificant levels of B cells or monocytes were identified. Cells bearing the human T cell receptors were found. Spleen cells from mice injected with human cells immune to CMV proliferated to CMV antigens (SI = 17). Similar proliferative studies after immunization of SCID mice with recombinant gp120 and other HIV antigens are in progress. CONCLUSION: Xenochimeric SCID mice may be a useful in vivo model for studying immune responses of human cells to HIV and other viral antigens.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- Antigens, CD8
- Antiviral Agents
- B-Lymphocytes
- CD4-Positive T-Lymphocytes
- Female
- Flow Cytometry
- HIV Envelope Protein gp120
- HIV Infections
- Humans
- Lymphocytes
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred NOD
- Mice, SCID
- Models, Biological
- Receptors, Antigen, T-Cell
- Spleen
- immunology
Other ID:
UI: 102181851
From Meeting Abstracts