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Xenochimeric SCID mice as a possible model for human antiviral immunity.

Svedersky L, Roey S, Scillian J, Parslow T, Stites D; International Conference on AIDS.

Int Conf AIDS. 1990 Jun 20-23; 6: 193 (abstract no. Th.A.294).

Department of Laboratory Medicine, UCSF, San Francisco, California, USA

OBJECTIVE: To investigate the use of xenochimeric SCID mice as an in vivo model for human T and B cell immune responses to HIV antigens. METHODS: Male and female SCID mice, 6 to 12 weeks old, were used. One to 3 x 10(7) human peripheral blood lymphocytes, obtained from Ficoll-Hypaque density gradients of HIV seronegative and cytomegalovirus (CMV) antibody positive donors, were injected intraperitoneally. Spleen cells from groups consisting of at least 4 mice were combined and analyzed 1 to 12 weeks post-injection for their ability to proliferate to CMV after 5 days of culture. Splenocytes were analyzed by flow cytometry for the presence of human lymphocyte and monocyte markers. RESULTS: No human monocytes, T or B cells were found when spleen cells from mice injected with human cells 1, 2, 3 or 4 weeks previously were analyzed. When spleen cells from mice injected with human cells 6 to 13 weeks previously were used, a range of 12% to 55% CD4+ cells were found. In these experiments, less than 10% CD8+ and insignificant levels of B cells or monocytes were identified. Cells bearing the human T cell receptors were found. Spleen cells from mice injected with human cells immune to CMV proliferated to CMV antigens (SI = 17). Similar proliferative studies after immunization of SCID mice with recombinant gp120 and other HIV antigens are in progress. CONCLUSION: Xenochimeric SCID mice may be a useful in vivo model for studying immune responses of human cells to HIV and other viral antigens.

Publication Types:
  • Meeting Abstracts
  • AIDS Vaccines
  • Acquired Immunodeficiency Syndrome
  • Animals
  • Antigens, CD8
  • Antiviral Agents
  • B-Lymphocytes
  • CD4-Positive T-Lymphocytes
  • Female
  • Flow Cytometry
  • HIV Envelope Protein gp120
  • HIV Infections
  • Humans
  • Lymphocytes
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NOD
  • Mice, SCID
  • Models, Biological
  • Receptors, Antigen, T-Cell
  • Spleen
  • immunology
Other ID:
  • 10029490
UI: 102181851

From Meeting Abstracts

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