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Fluorescence Microscopy & Imaging Center

This section* is designed to help you gain a good conceptual understanding of the many techniques that are supported in the NIEHS Fluorescence Microscopy and Imaging Center. Below is a list of Web links that you can explore to learn more about many of these techniques. It is suggested that you familiarize yourself with the first three in this list before delving into the more advanced topics. If you are already a FMIC user, you might be able to find more technical information on the confocal file server.


Conventional fluorescence microscopy

Multi-photon (2-photon) excitation microscopy

DIC (Differential Interference Contrast, also called Nomarski) microscopy

Quantitative colocalization image analysis

3D deconvolution of microscope images

Localized photolysis and photoactivation

FRAP (Fluorescence Recovery After Photobleaching)

FRET (Förster Resonance Energy Transfer)

FLIM (Fluorescence Lifetime Imaging)

TIRF (Total Internal Reflection-induced Fluorescence)

FAIM (Fluorescence Anisotropy Imaging)

* The inclusion of Web sites by vendors does not indicate any endorsement of those vendors' products. Rather, these are just the best Web sites the center has found, after a brief Web search, to explain the concepts. Please contact the center if you have found better Web sites.

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Last Reviewed: June 05, 2007