U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M161
Tryptone Broth and Tryptone Salt Broths
(T1N0, T1N1, T1N3, T1N6, T1N8, T1N10)

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Trypticase
or tryptone		 10 g
NaCl 0, 10, 30, 60, 80, or 100 g
Distilled water 1 liter

Dissolve ingredients in distilled water.

For T1N0, add no NaCl.
For T1N1, use 10 g NaCl [1% (w/v) NaCl].
For T1N3, use 30 g NaCl/liter [3% (w/v) NaCl], etc.


Dispense into 16 x 125 mm screw-cap tubes. Tighten caps to maintain correct salt concentration in tube. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21