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Research Interests -- Functional Genomics and Microbial PhysiologyCurrent research projects in Dr. Brown’s laboratory include characterizing the dynamics of stress responses and genetic regulation in Zymomonas mobilis ZM4. We have developed a whole genome microarray that will be used in conjugation with other approaches such as metabolomic profiling and mutagenesis to elucidate the mechanisms and regulation of stress tolerance in strain ZM4. Dr. Brown has established a laboratory for controlled cultivation at ORNL that will allow control of, and log data for culture pH, temperature, level, dissolved oxygen tension (DOT) and thermal mass flow. Click here for a short video showing three of six 7.5L New Brunswick Scientific fermentors in action and here for some of the types of data we can collect in my laboratory. Dr. Brown and Dr. Phelps are preparing to interface a GC-MS system for online metabolite analysis, so check back for updates soon. The cultivation laboratory also contains aerobic and anaerobic Bioscreen C machines for growth and monitoring of 200 individual cultures at a time. Dr. Brown has recently fabricated several whole genome microarrays for Clostridium thermocellum 27405 (lead-PI, Dr. Jonathan Mielenz) and Desulfovibrio desulfuricans G20 (lead-PI, Dr. Tony Palumbo) and is presently analyzing transcriptomic and other data as part of a larger systems biology team at ORNL. Dr. Brown has also conducted integrated biochemical, genomics and proteomic studies on Shewanella oneidensis MR-1 (more details). A full list of whole genome microarray developed at ORNL (some originally developed by Dr. Jizhong Zhou) can be found here as well as a list of some of our genomics resources. Please contact Dr. Brown regarding prospective collaborative research opportunities. Education [ Top ]
Professional Experience [ Top ]
Selected Publications [ Top ]S. D. Brown, B. Raman, C. K. McKeown, S. P. Kale, Z. He, and J. R. Mielenz. Construction and evaluation of a Clostridium thermocellum 27405 whole-genome oligonucleotide microarray. 2006. Appl. Biochem. Biotechnol. In review. Chourey, K., M. R. Thompson, J. Morrell-Falvey, N. C. VerBerkmoes, S. D. Brown, M. Shah, J. Zhou, M. Doktycz, R. L. Hettich, and D. K. Thompson. 2006. Global molecular and morphological effects of 24-hour chromium (VI) exposure on Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 72: 6331-6344. S. D. Brown, M. R. Thompson, N. C. VerBerkmoes, K. Chourey, M. Shah, J. Zhou, R. L. Hettich and D. K. Thompson. Molecular Dynamics of the Shewanella oneidensis Response to Chromate Stress. 2006. Mol. Cell. Proteomics. 5: 1054-1071. A. B. Leaphart, D.K. Thompson, K. Huang, E. Alm, X. Wan, A. Arkin, S. D. Brown, L. Wu, T. Yan, X. Liu1, and J. Zhou. Transcriptome Analysis of Shewanella oneidensis Gene Expression in Response to Acidic and Alkaline pH Stress. 2006. J. Bacteriol. 188: 1633–1642. S. D. Brown, M. Martin, S. Deshpande, S. Seal, K. Huang, E. Alm, Y. Yang, L. Wu, T. Yan, X. Liu, A. Arkin J. Zhou, D. K. Thompson. Cellular Response of Shewanella oneidensis to Strontium Stress. 2006. Appl. Environ. Microbiol. 72: 890–900. Sullivan, J.T., Trzebiatowski, J.R., Cruickshank, R.W., Gouzy, J., Brown, S.D., Elliot, R.M., Fleetwood, D.J., McCallum, N.G., Rossbach, U., Stuart, G.S., Weaver, J.E., Webby, R.J., de Bruijn F.J., Ronson C.W. Comparative sequence analysis of the symbiosis island of Mesorhizobium loti strain R7A. 2002. J. Bacteriol. 184: 3086-95. Sullivan, J.T., Brown, S.D., Yocum, R.R., Ronson, C.W. 2001. The bio operon on the acquired symbiosis island of Mesorhizobium sp. strain R7A includes a novel gene involved in pimeloyl-CoA synthesis. Microbiol. 147: 1315-22. Links [ Top ]
October 2006 |
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