U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M9
Alkaline Peptone Salt Broth (APS)

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Peptone 10 g
NaCl 30 g
Distilled water 1 liter

Dissolve ingredients. Adjust pH so that value after sterilization is 8.5 ± 0.2. Dispense 10 ml into tubes. Autoclave 10 min at 121°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.


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