U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M85
Long-term Preservation Medium

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Yeast extract, 0.3% 3 g
Peptone 10 g
NaCl 30 g
Agar 3 g
Distilled water 1 liter

 

Heat to dissolve ingredients. Dispense 4 ml portions to 13 x 100 mm screw-cap tubes. Autoclave 15 min at 121°C. Cool and tighten caps for storage. No pH adjustment is necessary.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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