U.S. Food & Drug Administration
Center for
Food
Safety &
Applied
Nutrition
Bacteriological
Analytical
Manual Online
August 2001
|
|
M79
Letheen Broth (Modified)
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| Letheen broth |
25.7 g |
| Trypticase peptone |
5 g |
| Thiotone peptone |
10 g |
| Yeast extract |
2 g |
| Sodium bisulfite |
0.1 g |
| Distilled water |
1 liter |
Dispense 90 ml into screw-cap bottle. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.
Hypertext Source: Bacteriological Analytical
Manual, 8th Edition, Revision A, 1998.
Revisions: 2001-AUG-20 Formulation corrected, letheen broth amount corrected from 26.7 to
25.7.
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Hypertext updated by rim/cjm 2005-OCT-21