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M77
Lauryl Tryptose MUG (LST-MUG) Broth
Tryptose or trypticase | 20 g |
Lactose | 5 g |
K2HPO4 | 2.75 g |
KH2PO4 | 2.75 g |
NaCl | 5 g |
Sodium lauryl sulfate | 0.1 g |
4-Methylumbelliferyl- -D-glucuronide (MUG)* |
50 mg |
Distilled water | 1 liter |
Prepare lauryl tryptose broth and add MUG. Dissolve with gentle heat if necessary. Dispense 10 ml portions into 20 x 150 mm test tubes containing inverted 10 x 75 mm fermentation tubes. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.
*MUG may be obtained from several sources, e.g., HACH Co., Loveland, CO.
Hypertext updated by rim/cjm 2005-OCT-21