M75
Lactose-Gelatin Medium
(for Clostridium perfringens)
|
M75
Lactose-Gelatin Medium
(for Clostridium perfringens)
| Tryptose | 15 g |
| Yeast extract | 10 g |
| Lactose | 10 g |
| Phenol red (1% solution in 95% ethanol) | 5.0 ml |
| Gelatin | 120 g |
| Distilled water | 1 liter |
Heat to dissolve tryptose, yeast extract, and lactose in 400 ml water. Suspend gelatin in 600 ml water and heat at 50-60°C with agitation to dissolve. Mix 2 solutions. Adjust pH to 7.5 ± 0.2. Add phenol red and mix. Dispense 10 ml portions into 16 x 150 mm screw-cap tubes. Autoclave 10 min at 121°C. If not used within 8 h, deaerate by heating at 50-70°C for 2-3 h before use.
Hypertext updated by rim/cjm 2005-OCT-21
