U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M74
Lactose Broth

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Beef extract 3 g
Peptone 5 g
Lactose 5 g
Distilled water 1 liter

 

Dispense 225 ml portions into 500 ml Erlenmeyer flasks. After autoclaving 15 min at 121°C and just before use, aseptically adjust volume to 225 ml. Final pH, 6.9 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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