U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M69
King's B Medium

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Proteose peptone No. 3 20 g
Glycerol, C.P. 10 ml
K2HPO4 1.5 g
MgSO4 1.5 g
Agar 15 g
Distilled water 1 liter

Add all ingredients except MgSO4. Heat with agitation to dissolve agar. Adjust pH to 7.2 ± 0.2. Add MgSO4 slowly and mix. Dispense 4 ml portions to 13 x 100 tubes. Autoclave 15 min at 121°C.

 

Incline tubes to give half butt and half slant.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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