U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M68
Iron Milk Medium (Modified)

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Fresh whole milk 1 liter
Ferrous sulfate.7H2O 1 g
Distilled water 50 ml

Dissolve ferrous sulfate in 50 ml distilled water. Add slowly to 1 liter milk and mix with magnetic stirrer. Dispense 11 ml medium into 16 x 150 mm culture tubes. Autoclave 12 min at 118°C.

 

Prepare fresh medium before use.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21