U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M61
Hektoen Enteric (HE) Agar

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Caution!!
Storage 1 day only

Peptone 12 g NaCl 5 g
Yeast extract 3 g Sodium thiosulfate 5 g
Bile salts No. 3 9 g Ferric ammonium citrate 1.5 g
Lactose 12 g Bromthymol blue 0.065 g
Sucrose 12 g Acid fuchsin 0.1 g
Salicin 2 g Agar 14.0 g
Distilled water 1 liter

 

Heat to boiling with frequent agitation to dissolve. Boil no longer than 1 min. Do not overheat. Cool in water bath. Pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let dry 2 h with lids partially removed. Final pH, 7.5 ± 0.2. Do not store more than 1 day.

 




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21