U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M60
Heart Infusion (HI) Broth and Agar (HIA)
(for Vibrio)

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Beef heart, infusion from 500 g 1 liter
Tryptose 10 g
NaCl 5 g

Dissolve ingredients and dispense into tubes.

For halophilic Vibrio spp. add an extra 15 g NaCl per liter.


Autoclave 15 min at 121°C. Final pH, 7.4 ± 0.2.

For heart infusion agar, add 15 g agar/liter and boil to dissolve before dispensing and sterilizing.

Commercially available from Difco.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21