U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M59
Heart infusion agar (HIA)
(Difco)

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Beef heart infusion (Difco) 500 g
Tryptose 10 g
NaCl 5 g
Agar 15 g
Distilled water 1 liter

Or

Blood Agar Base No. 2 (Difco) 1 liter
Proteose peptone 15 g
Liver digest 2.5 g
Yeast extract 5 g
NaCl 5 g
Agar 12 g

Autoclave either base at 121°C for 15 min. If preparing blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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