U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M20a
Blood Agar Base
(commercially available blood agar base may be used)

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Infusion from beef heart 500 g
Tryptose 10 g
NaCl 5 g
Agar 15 g
Sheep blood
(sterile, defibrinated)		 50 ml
Distilled water 1 liter

 

 

Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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