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M185
Malt Agar (MA)
Malt extract, powdered | 20.0 g |
Agar | 20.0 g |
Distilled water | 1.0 liter |
Mix ingredients, steam to dissolve agar and sterilize for 15 min at 121°C. Temper medium to 45°C and pour plates under aseptic conditions.
To prepare slants dispense 5-6 ml of steamed medium (before autoclaving) into each of several 16 x 125 mm screw-cap tubes, loosely cap tubes and sterilize as above. After autoclaving lay tubes in a slanting position and let them cool.
This medium is recommended as a general maintenance medium.
Hypertext updated by rim/cjm 2005-OCT-21