U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M176
Vogel-Johnson (VJ) Agar

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Trypticase 10 g
Yeast extract 5 g
Mannitol 10 g
K2HPO4 5 g
Lithium chloride 5 g
Glycine 10 g
Phenol red 0.025 g
Agar 15 g
Distilled water 1 liter

 

 

Heat with agitation to dissolve agar. Autoclave 15 min at 121°C. Cool to 50°C. Add 20 ml Chapman tellurite solution, 1%, commercially available from Difco Laboratories, Detroit, MI. Mix and pour into plates. Final pH, 7.2 ± 0.2.

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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