Base

Prepare Nutrient Agar (M112). Dispense 100 ml portions into 170 ml bottles. Autoclave 15 min at 121°C. Cool to 48°C.

Tyrosine suspension

Suspend 0.5 g L-tyrosine in 10 ml distilled water in 20 x 150 mm culture tube. Mix thoroughly with Vortex mixer. Autoclave 15 min at 121°C.

Final medium

Combine 100 ml base with sterile tyrosine suspension. Mix thoroughly by gently inverting bottle 2 or 3 times. Aseptically dispense 3.5 ml into 13 x 100 mm tubes with frequent mixing. Slant tubes and cool rapidly to prevent separation of tyrosine.

Hypertext updated by rim/cjm 2005-OCT-21