U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M165
Tryptone Yeast Extract Agar

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Tryptone 10 g
Yeast extract 1 g
*Carbohydrate 10 g
Bromcresol purple 0.04 g
Agar 2 g
Distilled water 1 liter

Dissolve agar with heat and gentle agitation. Adjust pH to 7.0 ± 0.2. Fill 16 x 125 mm tubes 2/3 full. Autoclave 20 min at 115°C. Before use, steam medium 10-15 min. Solidify by placing tubes in ice water.

 

*Glucose and mannitol are the carbohydrates used for identification of Staphylococcus aureus.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21