U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M160
Trypticase Soy-Tryptose Broth

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Trypticase soy broth
(commercial, dehydrated)		 15 g
Tryptose broth
(commercial, dehydrated)		 13.5 g
Yeast extract 3 g
Distilled water 1 liter

 

Dissolve ingredients in 1 liter water. Heat gently to dissolve. Dispense 5 ml portions into 16 x 150 mm test tubes. Autoclave 15 min at 1210C. Final pH, 7.2 1 0.2.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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