U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M159
Trypticase Soy-Sheep Blood Agar

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Prepare Trypticase Soy Agar (M152). Sterilize as recommended and cool to 50°C. Add 5 ml defibrinated sheep blood to 100 ml agar. Mix and dispense 20 ml portions to 15 x 100 mm petri dishes. (Commercial trypticase soy-blood agar plates are satisfactory.)



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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