U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

B acteriological A nalytical M anual Online
January 2001

M155
Trypticase (Tryptic) Soy Broth (TSB)
with Glycerol

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Trypticase peptone (tryptone) 17 g
Phytone peptone (soytone) 3 g
NaCl 15 g
K2HPO4 2.5 g
Glycerol 240 ml
Distilled water 760 ml

 


Suspend ingredients in distilled water and heat to dissolve. Dispense into bottles or vials. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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