U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M148
Toluidine Blue-DNA Agar

(Return to Table of Contents)


Deoxyribonucleic acid (DNA) 0.3 g
Agar 10 g
CaCl2 (anhydrous) 1.1 mg
NaCl 10 g
Toluidine blue O 0.083 g
Tris (hydroxymethyl) aminomethane 6.1 g
Distilled water 1 liter

 

Dissolve Tris (hydroxymethyl) aminomethane in 1 liter distilled water. Adjust pH to 9.0. Add the remaining ingredients except toluidine blue O and heat to boiling to dissolve. Dissolve toluidine blue O in medium. Dispense to rubber-stoppered flasks. Sterilization is not necessary if used immediately. The sterile medium is stable at room temperature for 4 months and is satisfactory after several melting cycles.


 

Storage room temperature
Shelf life 4 months


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Home   |   B A M   |   B A M Media   |   Bad Bug Book

Hypertext updated by rim/cjm 2005-OCT-21