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M145
Tetrathionate (TT) Broth
Polypeptone | 5 g |
Bile salts | 1 g |
Calcium carbonate | 10 g |
Sodium thiosulfate.5H2O | 30 g |
Distilled water | 1 liter |
 
Suspend ingredients in 1 liter distilled water, mix, and heat to boiling. DO NOT AUTOCLAVE. (Precipitate will not dissolve completely.) Cool to less than 45°C. Store at 5-8°C. Final pH, 8.4 ± 0.2.
Potassium iodide | 5 g |
Iodine, resublimed | 6 g |
Distilled water, sterile | 20 ml |
Dissolve potassium iodide in 5 ml sterile distilled water. Add iodine and stir to dissolve. Dilute to 20 ml.
 
 
Brilliant green dye, sterile | 0.1 g |
Distilled water, sterile | 100 m1 |
On day of use, add 20 ml I2-KI solution and 10 ml brilliant green solution to 1 liter base. Resuspend precipitate by gentle agitation and aseptically dispense 10 ml portions into 20 x 150 or 16 x 150 mm sterile test tubes. Do not heat medium after addition of I2-KI and dye solutions.
Hypertext updated by rim/cjm 2005-OCT-21