U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M141
Spray's Fermentation Medium
(for Clostridium perfringens)

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Tryptone 10 g
Neopeptone 10 g
Agar 2 g
Sodium thioglycollate 0.25 g

Dissolve all ingredients except agar and adjust pH to 7.4 ± 0.2. Add agar and heat with agitation to dissolve. Dispense 9 ml portions into 16 x 125 mm tubes. Autoclave 15 min at 121 °C. Before use, heat in boiling water or flowing steam for 10 min. Add 1 ml of sterile 10% carbohydrate solution to 9 ml base.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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