|
M118a
PALCAM Listeria Selective Agar
Peptone | 23 g |
Starch | 1 g |
NaCl | 5 g |
Columbia agar | 13 g |
Mannitol | 10 g |
Ferric ammonium citrate | 0.5 g |
Esculin (aesculin) | 0.8 g |
Dextrose (glucose) | 0.5 g |
Lithium chloride | 15.0 g |
Phenol red | 0.08 g |
Distilled water | 1000 ml |
Polymyxin B sulfate | 10 mg |
Acriflavin | 5 mg |
Ceftazidine | 20 mg |
Distilled water | 2 ml |
To make 500 ml of medium, weigh 34.4 g basal medium powder (all ingredients except the three selective agents) and suspend in 500 ml distilled water. Sterilize by autoclaving at 121°C for 15 min. Dissolve the selective agent supplement mixture in sterile distilled water at 17.5 mg/ml and filter sterilize. Add 1 ml selective agent supplement solution to 500 ml sterile basal medium that has been cooled to 50°C. Mix gently and pour plates. Final pH, 7.2 ± 0.1.
*NOTE: The basal medium powder and lyophilized supplement mixture are available commercially; follow the manufacturer's instructions for preparing the medium.
Hypertext updated by rim/kwg/cjm 2005-OCT-21