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M110
Nitrate Reduction Medium and Reagents
Prepare nitrate broth (M108) from nutrient broth (M114) containing 1.0 g/liter potassium nitrate.
Reagent A. Dissolve 0.5 g -naphthylamine
(a carcinogen) in 100 ml 5 N acetic acid by gently heating. Prepare 5 N acetic acid by
adding distilled water to 28.7 ml glacial acetic acid (17.4 N) to give final volume of 100
ml.
Reagent B. Dissolve 0.8 g sulfanilic acid in 100 ml 5 N acetic acid by gently heating.
Reagent C. Dissolve 1 g -naphthol in 200 ml acetic acid.
Zinc powder.
Cadmium reagent. Place zinc rods in 20% solution of cadmium sulfate for several hours. Draw off precipitated cadmium and add to 1 N HCl.
Hypertext updated by rim/cjm 2005-OCT-21