M10a
ALOA (Agar Listeria Ottavani & Agosti) Medium
|
M10a
ALOA (Agar Listeria Ottavani & Agosti) Medium
| Meat peptone | 18 g |
| Tryptone | 6 g |
| Yeast extract | 10 g |
| Sodium pyruvate | 2 g |
| Glucose | 2 g |
| Magnesium glycerophosphate | 1 g |
| Magnesium sulphate | 0.5 g |
| Sodium chloride | 5 g |
| Lithium chloride | 10 g |
| Disodium hydrogen phosphate anhydrous | 2.5 g |
| 5-bromo-4-chloro-3-indolyl-ß-D-glucopyranoside | 0.05 g |
| Agar, according to gelation-strength | 12 g to 18 g |
| Water, according to volume of fungistat supplement | 925-930 ml |
Dissolve the dehydrated components or dehydrated complete base in the water by boiling. Adjust the pH, if necessary, so that after sterilization it is 7.2 ± 0.2.
Sterilize by autoclaving for 15 minutes at 121°C.
Supplements: Dissolve 2 g of L-
-Phosphatidylinositol (Sigma P 6636) in 50 ml of cold
distilled water.
Stir for about 30 minutes until a homogeneous suspension is obtained.
Autoclave at 121°C for 15 minutes. Cool to 48-50°C.
Dissolve the following compounds individually in the specified solvents and filter-sterilize the solutions: 0.02 g nalidixic acid (sodium salt) in 5 ml of water; 0.02 g of ceftazidime in 5 ml of water; 76700 U of polymyxin B in 5 ml of water; 0.05 g of cycloheximide in 2.5 ml of ethanol and add 2.5 ml of water; 0.01 g of amphotericin B in a mixture of 2.5 ml HCl (1 M) and 7.5 ml dimethylformamide.
Complete medium: Agar base 925 ml (or 930 ml); Nalidixic Acid solution; 5 ml; Ceftazidime
solution, 5 ml; Cycloheximide solution, 5 ml (or Amphotericin B solution 10 ml); Polymyxin B solution,
5 ml; L--Phosphatidylinositol solution, 50 ml.
This medium is available commercially as the ready-to-use agar medium.
Advisory for BAM Users on Reported Supply Problems for Cycloheximide
